annealing temperature การใช้

• In consequence the bonding strength rises with increasing annealing temperatures.
• The melting point of the primer sets the upper limit on annealing temperature.
• The earliest steps of a touchdown polymerase chain reaction cycle have high annealing temperatures.
• The annealing temperature during a polymerase chain reaction determines the specificity of primer annealing.
• The reduction of the required annealing temperature can be achieved by different pretreatments such as:
• Such annealing also crystallizes BN, the size of the crystallites increasing with the annealing temperature.
• The primers have all been optimised using gradient PCR to find the right annealing temperature and number of cycles.
• The 2nd generation of UK gas-cooled reactors, the AGRs, also operate above the annealing temperature of graphite.
• The reactor is operated above the Wigner annealing temperature so that the graphite does not accumulate dangerous amounts of Wigner energy.
• Touchdown increases specificity of the reaction at higher temperatures and increases the efficiency towards the end by lowering the annealing temperature.
• Gallium s behaviour does not seem to change after subsequent meltings and solidifications, but does depend on the annealing temperature that it is heated to.
• In PCR experiments, the GC-content of annealing temperature to the template DNA . A higher GC-content level indicates a higher melting temperature.
• However, that being said, if you are getting " contaminated " looking lanes, it's most likely the primers and the annealing temperature.
• :You can vary annealing temperature, length of annealing or extension time per cycle, magnesium concentration, Mg : dNTP ratio, Mg : salt ratio.
• For example, 10 % final concentration of DMSO in the PCR mixture with Phusion decreases primer annealing temperature ( i . e . primer melting temperature ) by.
• The annealing temperature is decreased in increments for every subsequent set of cycles ( the number of individual cycles and increments of temperature decrease is chosen by the experimenter ).
• The origin of these high coercivity values has been interpreted as a consequence of the subparticle structure induced by the different particle and crystallite size growth rates at increasing annealing temperature.
• Techniques for avoiding devitrification include cleaning the glass surfaces of dust or unwanted residue, and allowing rapid cooling once the piece reaches the desired temperature, until the temperature approaches the annealing temperature.
• Because heating of a sample above the annealing temperature causes the fission damage to heal or anneal, the technique is useful for dating the most recent cooling event in the history of the sample.
• Another approach is to immerse the case mouth in a molten alloy of lead that is at the desired annealing temperature for a few seconds, then quickly shake off the lead and quench the case.